Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Trp53

Cell type

Cell type Class
Neural
Cell type
Astrocytes
MeSH Description
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Attributes by original data submitter

Sample

source_name
Primary cortical astrocytes
antibody
p53 (1C12) CST
cell type
Primary cortical astrocytes
genotype
Trp53 KO
age
post natal day 1
Sex
female

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Astrocytes were fixed for chromatin isolation per Schmidl et l., Nature Methods 10:963. Chipmentation - Chromatin isolation with the indicated antibody followed by tagmentation with Tn5 as per Schmidl et l., Nature Methods 10:963.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

mm10

Number of total reads
18606379
Reads aligned (%)
90.2
Duplicates removed (%)
53.4
Number of peaks
135 (qval < 1E-05)

mm9

Number of total reads
18606379
Reads aligned (%)
90.2
Duplicates removed (%)
53.2
Number of peaks
94 (qval < 1E-05)

Base call quality data from DBCLS SRA