Antigen

Antigen Class

TFs and others

Antigen

Trp53

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

Primary cortical astrocytes

antibody

p53 (1C12) CST

cell type

Primary cortical astrocytes

genotype

Trp53 KO

age

post natal day 1

Sex

female

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Astrocytes were fixed for chromatin isolation per Schmidl et l., Nature Methods 10:963. Chipmentation - Chromatin isolation with the indicated antibody followed by tagmentation with Tn5 as per Schmidl et l., Nature Methods 10:963.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

mm10

Number of total reads

18606379

Reads aligned (%)

90.2

Duplicates removed (%)

53.4

Number of peaks

135 (qval < 1E-05)

mm9

Number of total reads

18606379

Reads aligned (%)

90.2

Duplicates removed (%)

53.2

Number of peaks

94 (qval < 1E-05)